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991.
J. T. Watson R. E. Ritzmann 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1997,182(1):23-33
We have combined kinematic and electromyogram (EMG) analysis of running Blaberus discoidalis to examine how middle and hind leg kinematics vary with running speed and how the fast depressor coxa (Df) and fast extensor
tibia (FETi) motor neurons affect kinematic parameters. In the range 2.5–10 Hz, B. discoidalis increases step frequency by altering the joint velocity and by reducing the time required for the transition from flexion
to extension. For both Df and FETi the timing of recruitment coincides with the maximal frequency seen for the respective
slow motor neurons. Df is first recruited at the beginning of coxa-femur (CF) extension. FETi is recruited in the latter half
of femur-tibia (FT) extension during stance. Single muscle potentials produced by these fast motor neurons do not have pronounced
effects on joint angular velocity during running. The transition from CF flexion to extension was abbreviated in those cycles
with a Df potential occurring during the transition. One effect of Df activity during running may be to phase shift the beginning
of joint extension so that the transition is sharpened. FETi is associated with greater FT extension at higher running speeds
and may be necessary to overcome high joint torques at extended FT joint angles.
Accepted: 24 May 1997 相似文献
992.
A total of 24 bacterial isolates able to grow on metal-working fluids were obtained from soil or metal-working fluids (both in-use and heavily contaminated fluids). Pure cultures of the isolates were tested for their ability to degrade a selection of components, including borate esters, phosphate ester, biocide and triethanolamine, typically found in synthetic metal-working fluids. All components, when present at a level equivalent to half that found in an in-use metal-working fluid, supported growth when utilised as the sole source of carbon and/or nitrogen. Each component was degraded by at least 50% by an individual isolate within 120 hours in batch liquid culture. 相似文献
993.
Alan B. Darlington Anna Halinska James F. Dat T. J. Blake 《Trees - Structure and Function》1997,11(4):223-228
Plant responses to saturation vapour pressure deficit (SVPD) were studied by subjecting black spruce [Picea mariana (Mill) B.S.P.] and jack pine seedlings (Pinus banksiana Lamb.) to humid (0.3 – 0.8 kPa) or dry (2.0 – 2.5 kPa SVPD) regimes for 4 weeks using a computer-controlled environmental
system to control diurnal variation in SVPD. Dry matter accumulation in needles was not altered by increasing SVPD. However,
root growth declined by 60% which increased shoot to root ratio and reduced total seedling dry weight in both black spruce
and jack pine. Relative growth rate of jack pine also declined to about half the rate of plants grown under humid conditions.
In situ root marking studies showed that the decline in root growth of jack pine under the high SVPD was the result of reduced
lateral root initiation, whereas root elongation was unaffected by humidity. A 4-week exposure to dry air increased abscisic
acid (ABA) levels in needles, but not roots, of jack pine whereas ABA levels in black spruce were not altered. A short (3-day)
exposure failed to increase needle ABA levels in either species. These results suggest that the responses of conifers to dry
air were not the result of ABA accumulation.
Received: 24 March 1996 / Accepted: 30 May 1996 相似文献
994.
995.
996.
Tyrosine phosphorylation of a 22-kDa protein is correlated with transformation by Rous sarcoma virus 总被引:26,自引:0,他引:26
J R Glenney 《The Journal of biological chemistry》1989,264(34):20163-20166
Recent studies from this laboratory have identified novel cytoskeletal proteins that are phosphorylated on tyrosine in vivo in Rous sarcoma virus-transformed chick fibroblasts (Glenney, J. R., Jr., and Zokas, L. (1989) J. Cell Biol. 108, 2401-2408). In the present report, the phosphorylation of these proteins was examined in cells expressing the nonmyristylated mutants of src that are not transformed. A good correlation was found between transformation and the tyrosine phosphorylation of a 22-kDa protein. Tyrosine phosphorylation of the 22-kDa protein was reduced more than 95% in cells expressing the nonmyristylated mutants of src. Size fractionation revealed that the 22-kDa phosphoprotein in transformed chick fibroblasts is found in a Mr 150,000 complex. Monoclonal antibodies were used to screen various chicken tissues where the 22-kDa protein was found at high levels in muscle and lung with low levels in epithelial cells and brain. The 22-kDa protein becomes an excellent candidate for a mediator of transformation by the tyrosine kinase class of oncogenes. 相似文献
997.
N Avissar J C Whitin P Z Allen D D Wagner P Liegey H J Cohen 《The Journal of biological chemistry》1989,264(27):15850-15855
Human plasma glutathione peroxidase (GSHPx) has been shown to be a glycosylated selenoprotein distinct enzymatically, structurally, and antigenically from known cellular glutathione peroxidases. The extracellular location of the enzyme and the fact that it is glycosylated suggested that it is a secreted protein. Utilizing mutually non-cross-reactive antibodies to human cellular and plasma GSHPx, we conducted a search to determine the tissue of origin for plasma GSHPx. The cells screened were endothelial cells because they are the main source of extracellular superoxide dismutase, HL-60 cells (myeloid cell line) because they are the main source of extracellular H2O2, and Hep G2 cells (hepatic cell line) because they are the source of many plasma proteins. Human umbilical vein endothelial cells were metabolically labeled with either [35S]methionine or [75Se]selenious acid, and HL-60 cells and Hep G2 cells were metabolically labeled with [75Se]selenious acid. Proteins were immunopurified from the labeled cells and their media with either anti-red blood cell (RBC) GSHPx IgG or with anti-plasma GSHPx IgG. Utilizing anti-RBC GSHPx IgG, only the cellular form of the enzyme was precipitated from all the cells tested but not from their media. When anti-plasma GSHPx IgG was applied to the cells and their media, a selenoprotein was precipitated only from the media of Hep G2 cells. When Hep G2 cells were incubated in the presence of the carboxylic ionophore monensin, an intracellular selenoprotein could be detected using anti-plasma GSHPx IgG. The precipitation of the cellular form from all three cell types was partially inhibited by preincubation of the anti-RBC GSHPx IgG with purified RBC GSHPx while the precipitation of the selenoprotein from the medium of Hep G2 cells by anti-plasma GSHPx IgG was prevented by preincubation of the antibody with purified plasma GSHPx. We suggest that plasma GSHPx is synthesized by and secreted from hepatic cells. This is, to the best of our knowledge, the only known selenoprotein with a defined function that has been shown to be synthesized for secretion by mammalian cells. 相似文献
998.
J Walleczek B Redl M St?ffler-Meilicke G St?ffler 《The Journal of biological chemistry》1989,264(7):4231-4237
We have investigated the protein-protein cross-links formed within the 50 S subunit of the Escherichia coli ribosome using 2-iminothiolane as the cross-linking reagent. The members of the cross-links have been identified by immunoblotting from one-dimensional and two-dimensional diagonal sodium dodecyl sulfate-polyacrylamide gels using antisera specific for the individual ribosomal proteins. This method also allowed a quantitation of the yield of cross-linking for each cross-link. A total of 14 cross-links have been identified: L1-L33, L2-L9, L2-L9-L28, L3-L19, L9-L28, L13-L21, L14-L19, L16-L27, L17-L30, L17-L32, L19-L25, L20-L21, L22-L32, and L23-L34. Our results are compared with those of Traut and coworkers (Traut, R. R., Tewari, D. S., Sommer, A., Gavino, G. R., Olson, H. M., and Glitz, D. G. (1986) in Structure, Function and Genetics of Ribosomes (Hardesty, B. and Kramer, G., eds) pp. 286-308, Springer-Verlag, New York). Our cross-linking data allow us to propose the approximate locations of eight proteins of the 50 S ribosomal subunit that so far have not been localized by immunoelectron microscopy and they thus contribute considerably to our knowledge of ribosome structure. 相似文献
999.
1000.